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According to the reality of outpatient clinic service,expanding the service items,laying off solid foundation,making the working procedures more concrete in details and establishing suitable polices,the professionalization of specialists and special diseases in outpatient clinic of multiple branches hospital,even resources of outpatient clinic of multiple branches hospital,diversified service pattern of outpatient clinic,refined outpatient clinic service procedure,informationization of treatment procedure of outpatient service,and different requirements of people of different levels are realized.
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Objective To study the changes in interleukin-32 (IL-32) in rats with Klebsiella bacillus pneumonia and approach its significance. Methods Seventy-two Sprague-Dawley(SD)rats were divide into control group,model group and experimental group by the method of random digits table,then the experimental group was subdivided into 4 hours and 1,3 and 5 days experimental subgroups(each n=6). The rat model of Klebsiella bacillus pneumonia was established by injection of 0.3 mL Klebsiella bacterial suspension into the trachea. Before the establishment of the model in the experimental group,IL-32 inhibitory agent,protease activated receptor-2(PAR2) was injected into the abdominal cavity. After model establishment,at different time points,blood was collected via tail vein to observe the changes in serum levels of IL-32,tumor necrosis factor-α(TNF-α),IL-6 and IL-8 in all the groups. The lungs were removed and stained with hematoxylin-eosin(HE)method to investigate the histopathological changes of the lung tissues under the light microscope. Results Compared to the control group, with the prolongation of time the levels of IL-32,TNF-α,IL-8 and IL-6 were increased gradually in the model group,and reached their peaks at 3 days〔IL-32(ng/L):84.40±28.24 vs. 18.57±3.86,t=5.544,P=0.002;TNF-α(ng/L):79.27±14.64 vs. 17.82±3.86, t=9.994, P=0.000;IL-8(ng/L):55.85±10.90 vs. 16.66±3.76,t=8.544, P=0.000;IL-6(ng/L):56.65±2.57 vs. 28.48±2.11,t=19.693,P=0.000〕;PAR2 could inhibit above indexes significantly,there was statistical difference at 3 days compared with the model group〔IL-32(ng/L):54.13±6.68 vs. 84.40±28.24,t=2.560,P=0.046;TNF-α(ng/L):49.12±3.56 vs. 79.27±14.64,t=4.901,P=0.003;IL-8 (ng/L):22.95±2.52 vs. 55.85±10.90,t=7.204,P=0.000;IL-6(ng/L):36.49±2.63 vs. 56.65±2.57,t=13.443, P=0.000〕. Under the light microscope,the inflammatory changes in the lung tissue in experimental group were milder than those in the model group. Conclusion As a pro-inflammatory cytokine,IL-32 can induce the production of TNF-α,IL-6 and IL-8,and the inhibition of IL-32 production may play a role in suppression of the development of Klebsiella bacillus pneumonia.
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Objective To observe the therapeutic effect of hyaluronic sodium product (SHP) on osteoarthritis(OA).Methods Three hundreds and forty-eight cases were diagnosed as OA and five hundred-eighty knee joints damage caused by OA were treated by intracavitary injection of SHP once a week for three consecutive weeks.The changes of the number of patients with clinical symptoms (joint pain,swelling of joint and with or without difficulty in up and down stairs) and the laboratory test results were compared before and after the treatment.Results It showed that there was a significant difference of the changes of the number of patients with clinical symptoms and laboratory test results before and after the treatment,(P
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AIM: To study the anti-inflammatory effect of the Antivirus Oral Liquid(AOL) in experimental rabbit conjunctivitis model. METHODS: Rabbit's staphococcus aureus conjunctivitis models and cantharides tincture conjunctivitis models were made to observe different groups of animals' symptom of eye stimulation,negative rate of bacteria and conjunctiva histopathology.And then AOL compared with the effect of amphemycin gutta or Shumu(gutta.) RESULTS: It showed that AOL possessed significant anti-inflammatory effect on bacterial conjunctivitis.The negative rate of bacteria was more than 90% after being given AOL for 4 days successively.Compared with the models group,the hyperemia and edemda were markedly relieved.The result showed that AOL possessed significant anti-inflammatory effect on irritable conjunctivitis.The hyperemia and edema were markedly relieved at the second day after being given AOL,and the inflammatory symptoms had almost disappeared after being given AOL for 4 days successively. CONCLUSION: AOL has a good effect on the therapy of staphylococcus aureus conjunctivitis and cantharides tincture.